Publications

Leynen N, Van Bellegem F, Wouters A, Bove H, Ploem JP, Thijssen E, Langie SAS, Carleer R, Ameloot M, Artois T, Smeets K. In vivo Toxicity Assessment of Silver Nanoparticles in Homeostatic versus Regenerating Planarians. Nanotoxicology. 2019 Feb 14:1-16. doi: 10.1080/17435390.2018.1553252. [Epub ahead of print] PubMed PMID: 30760077.

Van Roten A, Barakat AZA, Wouters A, Tran TA, Mouton S, Noben JP, Gentile L, Smeets K. A carcinogenic trigger to study the function of tumor suppressor genes in Schmidtea mediterranea. Dis Model Mech. 2018 Aug 16;11(9). pii: dmm032573. doi: 10.1242/dmm.032573. PubMed PMID: 29967069; PubMed Central PMCID: PMC6176991.

Stevens AS, Wouters A, Ploem JP, Pirotte N, Van Roten A, Willems M, Hellings N, Franken C, Koppen G, Artois T, Plusquin M, Smeets K. Planarians Customize Their Stem Cell Responses Following Genotoxic Stress as a Function of Exposure Time and Regenerative State. Toxicol Sci. 2018 Mar 1;162(1):251-263. doi: 10.1093/toxsci/kfx247. PubMed PMID: 29145667.

Evens R, Beenaerts N, Neyens T, Witters N, Smeets K, Artois T. Proximity of breeding and foraging areas affects foraging effort of a crepuscular, insectivorous bird. Sci Rep. 2018 Feb 14;8(1):3008. doi: 10.1038/s41598-018-21321-0. Sci Rep. 2018 Jun 20;8(1):9671. PubMed PMID: 29445120; PubMed Central PMCID: PMC5813100.

Stevens AS, Willems M, Plusquin M, Ploem JP, Winckelmans E, Artois T, Smeets K. Stem cell proliferation patterns as an alternative for in vivo prediction and discrimination of carcinogenic compounds. Sci Rep. 2017 May 3;7:45616. doi: 10.1038/srep45616. PubMed PMID: 28466856; PubMed Central PMCID: PMC5413882.

Kole PJ, Löhr AJ, Van Belleghem F, Ragas A (2017). Wear and tear of tyres: a stealthy source of microplastics in the environment. International journal of environmental research and public health, 14(10), 1265.

Löhr A, Savelli H, Beunen R, Kalz M, Ragas A, Van Belleghem F (2017). Solutions for global marine litter pollution. Current opinion in environmental sustainability, 28, 90-99.

Plusquin M, De Mulder K, Van Belleghem F, DeGheselle O, Pirotte N, Willems M, Cuypers A, Salvenmoser W, Ladurner P, Artois T, Smeets K. Toxic effects of cadmium on flatworm stem cell dynamics: A transcriptomic and ultrastructural elucidation of underlying mechanisms. Environ Toxicol. 2016 Oct;31(10):1217-28. doi: 10.1002/tox.22129. Epub 2015 Apr 6. PubMed PMID: 25846626.

Pieters N, Janssen BG, Dewitte H, Cox B, Cuypers A, Lefebvre W, Smeets K, Vanpoucke C, Plusquin M, Nawrot TS. Biomolecular Markers within the Core Axis of Aging and Particulate Air Pollution Exposure in the Elderly: A Cross-Sectional Study. Environ Health Perspect. 2016 Jul;124(7):943-50. doi: 10.1289/ehp.1509728. Epub 2015 Dec 15. PubMed PMID: 26672058; PubMed Central PMCID: PMC4937852.

Stevens AS, Pirotte N, Wouters A, Van Roten A, Van Belleghem F, Willems M, Cuypers A, Artois T, Smeets K. Redox-Related Mechanisms to Rebalance Cancer-Deregulated Cell Growth. Curr Drug Targets. 2016;17(12):1414-37. Review. PubMed PMID: 25944012.

Van Belleghem F, Van Roten A, Artois T, Smeets K (2016) Studying the mechanisms of Cd-induced stem cell proliferation and their role in tumor avoidance in Schmidtea mediterranea. Toxicology letters 258, S252

Stevens AS, Willems M, Plusquin M, Ploem JP, Artois T, Smeets K (2016) In vivo prediction and discrimination of carcinogenic compounds using Schmidtea mediterranea’s stem cell proliferation patterns. Toxicology letters 258, S65

Patent granted: Methods to defect carcinogens using flatworms WO 2016/146620 /EP15159158.3  A1 – Karen Smeets, An-Sofie Stevens, Michelle Plusquin, Tom Artois, Maxime Willems, Jean Paul Remon- filing date 15/03/2016

Pirotte N, Leynen N, Artois T, Smeets K. Do you have the nerves to regenerate? The importance of neural signalling in the regeneration process. Dev Biol. 2016 Jan 1;409(1):4-15. doi: 10.1016/j.ydbio.2015.09.025. Epub 2015 Nov 14. Review. PubMed PMID: 26586202.

Nair AR, Lee WK, Smeets K, Swennen Q, Sanchez A, Thévenod F, Cuypers A. Glutathione and mitochondria determine acute defense responses and adaptive processes in cadmium-induced oxidative stress and toxicity of the kidney. Arch Toxicol. 2015 Dec;89(12):2273-89. doi: 10.1007/s00204-014-1401-9. Epub 2014 Nov 12. PubMed PMID: 25388156.

Stevens AS, Pirotte N, Plusquin M, Willems M, Neyens T, Artois T, Smeets K. Toxicity profiles and solvent-toxicant interference in the planarian Schmidtea mediterranea after dimethylsulfoxide (DMSO) exposure. J Appl Toxicol. 2015 Mar;35(3):319-26. doi: 10.1002/jat.3011. Epub 2014 Jun 25. PubMed PMID: 24964768.

Pieters N, Janssen BG, Valeri L, Cox B, Cuypers A, Dewitte H, Plusquin M, Smeets K, Nawrot TS. Molecular responses in the telomere-mitochondrial axis of ageing in the elderly: a candidate gene approach. Mech Ageing Dev. 2015 Jan;145:51-7. doi: 10.1016/j.mad.2015.02.003. Epub 2015 Feb 28. PubMed PMID: 25736869.

Pirotte N, Stevens AS, Fraguas S, Plusquin M, Van Roten A, Van Belleghem F, Paesen R, Ameloot M, Cebrià F, Artois T, Smeets K. Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation. Oxid Med Cell Longev. 2015;2015:392476. doi: 10.1155/2015/392476. Epub 2015 Jun 9. PubMed PMID: 26180588; PubMed Central PMCID: PMC4477255.

Willems M, Stevens AS, Adriaens E, Plusquin M, Smeets K, Van Goethem F, Vanparys P, Janssen C, Remon JP (2015) An adult proliferation assay in the flatworm model Macrostomum lignano to predict the carcinogenicity of compounds. Applied In vitro Toxicology, 1(3), 213-219.

Nair AR*, Smeets K*, Keunen E, Lee W-K, Thévenod F, Van Kerkhove E, Cuypers A (2015) Renal cells exposed to cadmium in vitro and in vivo: normalizing gene expression data. Journal of Applied Toxicology. 35 (5), 478-484.

Remans T, Keunen E, Bex GJ, Smeets K, Vangronsveld J, Cuypers A. Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy. Plant Cell. 2014 Oct;26(10):3829-37. doi: 10.1105/tpc.114.130641. Epub 2014 Oct 31. PubMed PMID: 25361954; PubMed Central PMCID: PMC4247583.

Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT, Schjerling P, Day PJ, Abreu M, Aguado B, Beaulieu JF, Beckers A, Bogaert S, Browne JA, Carrasco-Ramiro F, Ceelen L, Ciborowski K, Cornillie P, Coulon S, Cuypers A, De Brouwer S, De Ceuninck L, De Craene J, De Naeyer H, De Spiegelaere W, Deckers K, Dheedene A, Durinck K, Ferreira-Teixeira M, Fieuw A, Gallup JM, Gonzalo-Flores S, Goossens K, Heindryckx F, Herring E, Hoenicka H, Icardi L, Jaggi R, Javad F, Karampelias M, Kibenge F, Kibenge M, Kumps C, Lambertz I, Lammens T, Markey A, Messiaen P, Mets E, Morais S, Mudarra-Rubio A, Nakiwala J, Nelis H, Olsvik PA, Pérez-Novo C, Plusquin M, Remans T, Rihani A, Rodrigues-Santos P, Rondou P, Sanders R, Schmidt-Bleek K, Skovgaard K, Smeets K, Tabera L, Toegel S, Van Acker T, Van den Broeck W, Van der Meulen J, Van Gele M, Van Peer G, Van Poucke M, Van Roy N, Vergult S, Wauman J, Tshuikina-Wiklander M, Willems E, Zaccara S, Zeka F, Vandesompele J. The need for transparency and good practices in the qPCR literature. Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697. PubMed PMID: 24173381.

Smeets K, Opdenakker K, Remans T, Forzani C, Hirt H, Vangronsveld J, Cuypers A. The role of the kinase OXI1 in cadmium- and copper-induced molecular responses in Arabidopsis thaliana. Plant Cell Environ. 2013 Jun;36(6):1228-38. doi: 10.1111/pce.12056. Epub 2013 Jan 30. PubMed PMID: 23278806.

Pieters N, Koppen G, Smeets K, Napierska D, Plusquin M, De Prins S, Van De Weghe H, Nelen V, Cox B, Cuypers A, Hoet P, Schoeters G, Nawrot TS. Decreased mitochondrial DNA content in association with exposure to polycyclic aromatic hydrocarbons in house dust during wintertime: from a population enquiry to cell culture. PLoS One. 2013 May 3;8(5):e63208. doi: 10.1371/journal.pone.0063208. Print 2013. PubMed PMID: 23658810; PubMed Central PMCID: PMC3643917.

Nair AR*, Degheselle O*, Smeets K*, Van Kerkhove E, Cuypers A. Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)? Int J Mol Sci. 2013 Mar 18;14(3):6116-43. doi: 10.3390/ijms14036116. PubMed PMID: 23507750; PubMed Central PMCID: PMC3634456.

Janssen BG, Munters E, Pieters N, Smeets K, Cox B, Cuypers A, Fierens F, Penders J, Vangronsveld J, Gyselaers W, Nawrot TS. Placental mitochondrial DNA content and particulate air pollution during in utero life. Environ Health Perspect. 2012 Sep;120(9):1346-52. doi: 10.1289/ehp.1104458. Epub 2012 May 24. PubMed PMID: 22626541; PubMed Central PMCID: PMC3440109.

Plusquin M, DeGheselle O, Cuypers A, Geerdens E, Van Roten A, Artois T, Smeets K. Reference genes for qPCR assays in toxic metal and salinity stress in two flatworm model organisms. Ecotoxicology. 2012 Mar;21(2):475-84. doi: 10.1007/s10646-011-0809-8. Epub 2011 Nov 12. PubMed PMID: 22080432.

Plusquin M, Stevens AS, Van Belleghem F, Degheselle O, Van Roten A, Vroonen J, Blust R, Cuypers A, Artois T, Smeets K. Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea. Int J Dev Biol. 2012;56(1-3):183-91. doi: 10.1387/ijdb.113485mp. PubMed PMID: 22451006.

Weyens N, Boulet J, Adriaensen D, Timmermans JP, Prinsen E, Van Oevelen S, D’Haen J, Smeets K, van er Lelie D, Taghavi S, Vangronsveld J (2012) Contrasting colonization and plant growth promoting capacity between wild type and a gfp-derivate of the endophyte Pseudomonas putida W619 in hybrid poplar. Plant and Soil 356 (1-2), 217-230.

Cuypers A, Remans T, Vangronsveld J, Smeets K (2012) Cadmium and oxidative stress. In: Encyclopedia of Metalloproteins (Eds. R.H. Kretsinger, E.A. Permyakov, V.M. Uversky).

Nawrot TS, Staessen JA, Roels H, Cuypers A, Smeets K, Vangronsveld J (2012) Health risks from cadmium. In: Encyclopedia of Metalloproteins (Eds. R.H. Kretsinger, E.A. Permyakov, V.M. Uversky). 

Nelissen K, Mulder M, Smets I, Timmermans S, Smeets K, Ameloot M, Hendriks JJ. Liver X receptors regulate cholesterol homeostasis in oligodendrocytes. J Neurosci Res. 2012 Jan;90(1):60-71. doi: 10.1002/jnr.22743. Epub 2011 Oct 3. PubMed PMID: 21972082.

Vanhoudt N, Cuypers A, Horemans N, Remans T, Opdenakker K, Smeets K, Bello DM, Havaux M, Wannijn J, Van Hees M, Vangronsveld J, Vandenhove H. Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part II: responses in the leaves and general conclusions. J Environ Radioact. 2011 Jun;102(6):638-45. doi: 10.1016/j.jenvrad.2011.03.013. Epub 2011 Apr 15. PubMed PMID: 21497426.

Vanhoudt N, Vandenhove H, Horemans N, Remans T, Opdenakker K, Smeets K, Bello DM, Wannijn J, Van Hees M, Vangronsveld J, Cuypers A. Unraveling uranium induced oxidative stress related responses in Arabidopsis thaliana seedlings. Part I: responses in the roots. J Environ Radioact. 2011 Jun;102(6):630-7. doi: 10.1016/j.jenvrad.2011.03.015. Epub 2011 Apr 13. PubMed PMID: 21492976.

Cuypers A*, Smeets K*, Ruytinx J, Opdenakker K, Keunen E, Remans T, Horemans N, Vanhoudt N, Van Sanden S, Van Belleghem F, Guisez Y, Colpaert J, Vangronsveld J. The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings. J Plant Physiol. 2011 Mar 1;168(4):309-16. doi: 10.1016/j.jplph.2010.07.010. Epub 2010 Sep 15. PubMed PMID: 20828869.

Ruttens A, Boulet J, Weyens N, Smeets K, Adriaensen K, Meers E, Van Slycken S, Tack F, Meiresonne L, Thewys T, Witters N, Carleer R, Dupae J, Vangronsveld J. Short rotation coppice culture of willows and poplars as energy crops on metal contaminated agricultural soils. Int J Phytoremediation. 2011;13 Suppl 1:194-207. PubMed PMID: 22046760.

Hendricks J, Nelissen K, Mulder M, Smets I, Timmermans S, Smeets K, Ameloot M (2011) Liver receptors regulate cholesterol homeostasis. Glia 59 (1) S54-S54.

Nawrot TS, Staessen JA, Roels HA, Munters E, Cuypers A, Richart T, Ruttens A, Smeets K, Clijsters H, Vangronsveld J. Cadmium exposure in the population: from health risks to strategies of prevention. Biometals. 2010 Oct;23(5):769-82. doi: 10.1007/s10534-010-9343-z. Epub 2010 Jun 3. Review. PubMed PMID: 20517707.

Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H, Opdenakker K, Nair AR, Munters E, Artois TJ, Nawrot T, Vangronsveld J, Smeets K. Cadmium stress: an oxidative challenge. Biometals. 2010 Oct;23(5):927-40. doi: 10.1007/s10534-010-9329-x. Epub 2010 Apr 2. Review. PubMed PMID: 20361350.

Nelissen K, Smeets K, Mulder M, Hendriks JJ, Ameloot M. Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR. J Neurosci Methods. 2010 Mar 15;187(1):78-83. doi: 10.1016/j.jneumeth.2009.12.018. Epub 2009 Dec 29. PubMed PMID: 20036692.

Semane B, Dupae J, Cuypers A, Noben JP, Tuomainen M, Tervahauta A, Kärenlampi S, Van Belleghem F, Smeets K, Vangronsveld J. Leaf proteome responses of Arabidopsis thaliana exposed to mild cadmium stress. J Plant Physiol. 2010 Mar 1;167(4):247-54. doi: 10.1016/j.jplph.2009.09.015. Epub 2009 Dec 11. PubMed PMID: 20005002.

Remans T, Opdenakker K, Smeets K, Mathysen D, Vangronsveld J, Cuypers A (2010) Metal-specific and NADPH oxidase dependent changes in lipoxygenase and NADPH oxidase gene expression in Arabidopsis thaliana exposed to cadmium or excess copper. Functional Plant Biology 37 (6), 532-544.

Weyens N, van der Lelie D, Artois T, Smeets K, Taghavi S, Newman L, Carleer R, Vangronsveld J. Bioaugmentation with engineered endophytic bacteria improves contaminant fate in phytoremediation. Environ Sci Technol. 2009 Dec 15;43(24):9413-8. doi: 10.1021/es901997z. PubMed PMID: 20000537.

Smeets K, Opdenakker K, Remans T, Van Sanden S, Van Belleghem F, Semane B, Horemans N, Guisez Y, Vangronsveld J, Cuypers A. Oxidative stress-related responses at transcriptional and enzymatic levels after exposure to Cd or Cu in a multipollution context. J Plant Physiol. 2009 Dec 15;166(18):1982-92. doi: 10.1016/j.jplph.2009.06.014. Epub 2009 Aug 26. PubMed PMID: 19709775.

Cuypers A, Smeets K, Vangronsveld J (2009) Heavy metal stress in plants. In: Plant stress biology: From Genomics to Systems Biology (ed H. Hirt), pp. 161-178, Wiley-VCH Verlag, Weinheim.

Vanhoudt N, Vandenhove H, Smeets K, Remans T, Van Hees M, Wannijn J, Vangronsveld J, Cuypers A. Effects of uranium and phosphate concentrations on oxidative stress related responses induced in Arabidopsis thaliana. Plant Physiol Biochem. 2008 Nov;46(11):987-96. doi: 10.1016/j.plaphy.2008.06.003. Epub 2008 Jun 13. PubMed PMID: 18640846.

Remans T, Smeets K, Opdenakker K, Mathijsen D, Vangronsveld J, Cuypers A. Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrations. Planta. 2008 May;227(6):1343-9. doi: 10.1007/s00425-008-0706-4. Epub 2008 Feb 14. PubMed PMID: 18273637.

Smeets K, Ruytinx J, Van Belleghem F, Semane B, Lin D, Vangronsveld J, Cuypers A. Critical evaluation and statistical validation of a hydroponic culture system for Arabidopsis thaliana. Plant Physiol Biochem. 2008 Feb;46(2):212-8. Epub 2007 Oct 9. PubMed PMID: 18024051.

Smeets K, Ruytinx J, Semane B, Van Belleghem F, Van Sanden S, Remans T, Vangronsveld J, Cuypers A (2008) Cadmium-induced transcriptional and enzymatic alterations related to oxidative stress. Environmental and Experimental Botany 63, 1-8.

Thijssen S, Cuypers A, Maringwa J, Smeets K, Horemans N, Lambrichts I, Van Kerkhove E. Low cadmium exposure triggers a biphasic oxidative stress response in mice kidneys. Toxicology. 2007 Jul 1;236(1-2):29-41. Epub 2007 Mar 31. PubMed PMID: 17499415.

Van Belleghem F, Cuypers A, Semane B, Smeets K, Vangronsveld J, d’Haen J, Valcke R. Subcellular localization of cadmium in roots and leaves of Arabidopsis thaliana. New Phytol. 2007;173(3):495-508. PubMed PMID: 17244044.

Semane B, Cuypers A, Smeets K, Van Belleghem F, Horemans N, Schat H, Vangronsveld J (2007) Cadmium responses in Arabidopsis thaliana: glutathione metabolism and antioxidative defence system. Physiologia Plantarum 129 (3), 519-521.

Smeets K, Cuypers A, Lambrechts A, Semane B, Hoet P, Van Laere A, Vangronsveld J. Induction of oxidative stress and antioxidative mechanisms in Phaseolus vulgaris after Cd application. Plant Physiol Biochem. 2005 May;43(5):437-44. Epub 2005 Apr 25. PubMed PMID: 15890519.